mRNA is always known for its twisted structure but if considered then its vaccine also has the same entangled pattern. In late, Robert Malone, an American virologist cum immunologist performed an indicating experiment.
The immunologist mainly combined the strands of the messenger Ribonucleic acid with some droplets of fat, so that molecular stew can be developed. The human cells are soaked within this genetic gumbo and absorbed the mRNA, which started the production of proteins from this fusion.
Soon, Malone, who is a graduate of Salk Institute for Biological Studies in La Jolla, California realized that accidentally he has achieved the far-reaching potential in medicines, and he also marked out a few of the notes, that was signed by him with proper dates.
On 11 January 1988, Robert noted that if the cells can lead to the production of protein from mRNA, delivered within them. However, it was also mentioned that it might be a way to treat RNA as a drug and another member of the Salk lab also approved of this postulation.
The experiment was done by Robert depicted that the embryos of the frog simply absorbed the mRNA. Hence, this was the first time when anyone could utilize the fat droplets to ease mRNA’s way within the living organism.
Notably, the experiments performed by Robert Malone were simply a start of stepping on towards the two most prior and profitable vaccines till today. The covid-19 vaccination and the mRNA-based one were provided to the million people worldwide. In 2021, the sales covered were about US $50 billion itself.
Considerably, the entire path for success was not just easy, the immunologist performed and made himself busy with the experiments. This experiment was drawn on the other different experts and the instability within the mRNA was noticeable. Additionally, each dosage of the vaccine is highly expensive as a drug as well as a vaccine.
There were many academic kabs and companies which in collation worked together. They had to toil hard and develop the correct formula of fats and other nucleic acids, which could be the main building blocks of the mRNA vaccines.
The messenger- RNA was basically developed during the procedure of transcript, in which an enzyme called RNA polymerase turns the gene to a primary transcript mRNA or pre- mRNA. This pre- mRNA also carries introns, regions that won’t be going for the coding to the last amino acid sequence.
All these were being thrown away during the process called RNA splicing, in which the exons are only left, and regions would be encoding the protein. The exon pattern has mature mRNA and this mature mRNA is further read by the ribosome, with the utilization of amino acids.
Furthermore, they are carried off by the transfer RNA which further leads to the production of protein by the ribosome. This entire process forms the chunk of the central dogma of molecular biology.
This central dogma perfectly elaborates the flow of genetic information within the biological system. On the other hand, the protein-coding portion of the coding spots might serve as the sequences in the pre RNA, on the regular basis.